Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Illustration of the effects of acetaminophen on glutathione, Download Scientific Diagram PDF] Evidence that Increased Acetaminophen use in Genetically Vulnerable Children Appears to be a Major Cause of the Epidemics of Autism, Attention Deficit with Hyperactivity, and Asthma Semantic Scholar Electrochemical evidences in oxidation of acetaminophen in the presence of glutathione and N acetylcysteine Chemical Communications (RSC Publishing) Xia & He Publishing The Hidden Impact of Acetaminophen Well Rooted